Micropara

• What type of microscope contains more than one magnifying lens?

  Compound microscope
• How much can a compound microscope magnify objects?

  Approximately a thousand times
• What is the main source of illumination for a compound microscope?

  Visible light
• What is another name for the compound microscope?

  Compound light microscope
• How many magnifying lens systems does a compound microscope consist of?

  Two magnifying lens systems
• What is the magnifying power of the ocular lens in a compound microscope?

  10x
• Where is the objective lens located in a compound microscope?

  Above the organism to be viewed
• What are the main components of a compound microscope?

  • Ocular lens or eyepiece
  • Revolving nose piece
  • Diopter adjustment
  • Body tube or head
  • Arm
  • Coarse adjustment
  • Fine adjustment
  • Objective lenses
  • Stage
  • Stage clips
  • Stage control
  • Aperture
  • On/off switch
  • Illuminator
  • Iris diaphragm
  • Condenser
  • Base
• What is the function of the ocular lens in a microscope?

  It allows the viewer to see the specimen
• Where is the revolving nosepiece located in a compound microscope?

  Above the stage
• What is the purpose of the diopter adjustment?

  To correct vision differences between eyes
• What does the body tube connect in a compound microscope?

  It connects the eyepiece to the objective lenses
• What is the function of the arm in a compound microscope?

  It connects the body tube to the base
• What does the coarse adjustment do in a microscope?

  It brings the specimen into general focus
• What is the purpose of the fine adjustment in a microscope?

  To increase the details of the specimen
• What are the objective lenses closest to in a compound microscope?

  Closest to the specimen
• How many objective lenses does a compound microscope typically have?

  3 to 5 objectives
• What is the stage in a compound microscope?

  Flat platform for the specimen
• What are stage clips used for in a microscope?

  To hold the slide in place
• What does the stage control do in a microscope?

  Moves the stage left or right
• What is the aperture in a compound microscope?

  Hole for light to reach the slide
• Where is the on/off switch located in a microscope?

  At the base of the microscope
• What is the function of the illuminator in a microscope?

  It serves as the light source
• What does the iris diaphragm do in a microscope?

  Adjusts the amount of light coming through
• What is the role of the condenser in a microscope?

  Focuses light onto the specimen
• What does the base of a microscope do?

  Supports the microscope
• What is the brightfield microscope used for?

  To visualize bacteria and fungi
• What is the maximum magnification of a brightfield microscope?

  1,000 to 1,500 times
• What is the limitation of a brightfield microscope regarding object size?

  Objects less than 0.2 µm cannot be visualized
• Why do most cells need to be stained in a brightfield microscope?

  To improve contrast for proper viewing
• How does a darkfield microscope illuminate specimens?

  It uses reflected light instead of transmitted light
• What does a specimen look like under a darkfield microscope?

  Bright against a dark background
• What type of specimens is a darkfield microscope ideal for?

  Unstained or transparent specimens
• What external details can a darkfield microscope examine?

  Outline or surface of the specimen
• What type of organisms can be viewed with a darkfield microscope?

  Spirochetes
• What principle does phase-contrast microscopy rely on?

  Differences in refractive indices and light waves
• Who introduced phase-contrast microscopy?

  Frits Zernike
• What is the purpose of phase-contrast microscopy?

  To produce high-contrast images of transparent specimens
• How does the differential interference contrast microscope differ from the phase-contrast microscope?

  It utilizes two beams of light instead of one
• What causes the contrasting colors in a differential interference contrast microscope?

  Prisms that split the light beam