Practicals

• The Aim of this practical is to investigate effect of pH on Amylase
• Amylase breaks down starch molecules into simple sugars
• 1. Place a drop of iodine solution into each well of a spotting tile
• 2. Get your 3 test tubes one including 2cm3 starch solution, another one of 2cm3 amylase solution and one more of 2cm3 of pH 5 buffer solution
• 3. Place all 3 test tubes in a water bath at 30 degrees Celsius, Leave them for 10 minutes to allow the solutions to reach the correct temperature
• 4. Now combine the three solutions into one test tube and mix with a stirring rod. Return to the waterbath and start a stopwatch
• 5. After thirty seconds use the stirring rod to transfer one drop of solution to a spotting tile that contains iodine
• 6. The iodine should turn blue/black showing that starch is present
• 7. Now every thirty seconds we put a drop of solution into an iodine spotting pile until the iodine remains orange
• 8. Repeat the whole experiment 7 times with different ph buffers like 6 7 and 8
• 9. Record your results on a table
• 10. Draw a graph of pH against time
• 11. Use the graph to find out what the optimum pH range is for amylase enzyme activity.