MICROSCOPY

• Zaccharias Janssen and Hans Janssen discovered the compound

  microscope.
• A microscope may be called compound when it uses two or more

  lenses.
• Anton Van Leeuwenhoek was able to make a simple microscope with a magnification so powerful that it can be enlarged 200 times.
• Anton Van Leeuwenhoek is the “Father of Microscopy”
• Microscope is an instrument used to magnify object that cannot be seen by the naked eye.
• Microscope is an essential tool in the study of the fine structure of an organism, especially cells.
• Simple microscope are composed of one lens and provide relatively low magnifying power (5x).
• Compound Microscope are composed of two or more lenses that provide powerful magnification.
• Electron Microscope uses electron beam as a source of illumination, can magnify up to 10,000,000 times.
• Mechanical Parts are the parts of the microscopes that support, adjust, connect, and move other parts.
• The Mechanical Parts of a microscope are:

  Base

  Arm

  Body Tube

  the ocular eyepiece and the objectives

  Draw Tube

  Stage

  Stage Clip

  Course Adjustment Screw

  Fine Adjustment Screw

  Revolving Nosepiece
• The Illuminating Parts are the parts that provide light.
• The Illuminating parts of a microscope are:

  Mirror

  Diaphragm

  Ocular/Eyepiece

  Objectives
• The Magnifying Parts are the parts that enlarge the object or specimen.
• The magnifying parts of a microscope are:

  Ocular/Eyepiece

  Objectives
• Low Power Objectives (LPO) that locates and enlarges the specimen 10x and its typical color is yellow.
• High Power Objectives (HPO) that enlarges the specimen 40x, 43x, or 45x and its typical color is blue.
• Oil Immersion Objectives (OIO) that enlarges the object 100x and its typical color is white.
• the lowest power lens is the shortest in length.
• Always keep both eye/s open.
• When using a microscope, everything is backward and upside down
• Objectives – aside from gathering light, they magnify the image
• Ocular/Eyepiece can magnify up to five times (5x) to ten times (10x).
• Objectives gather light
• Ocular/Eyepiece contains lenses, focuses light to the eye; where the observer peeps through
• Diaphragm regulates the amount of light striking the specimen.
• Mirror collects and reflects the light from the source to the specimen
• Revolving Nosepiece facilitates the shifting of the objectives
• Fine Adjustment Screw is used to focus finer detail or sharp focus of the object/ specimen being viewed
• Fine Adjustment Screw is used only when using High Power Objective
• Coarse Adjustment Screw is used to focus the object with Low Power Objective and moves the body tube faster to focus the specimen.
• Stage Clip is used to hold the slide in place.
• Stage is where the glass slide is placed
• Draw Tube is where the eyepiece is inserted.
• Body Tube is the connection between the ocular eyepiece and the objectives
• Arm supports the body tube and used for carrying the microscope.
• Base supports the entire weight of the microscope.