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biology
PAGs
PAG 4: enzyme-controlled reactions
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Created by
lucy dock
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Cards (9)
word equation for the action of a catalase
hydrogen peroxide
->
water
+
oxygen
how is the rate of catalase activity indicated?
by measuring the
volume
of
oxygen gas
released in a
fixed time
what is the function of potato in this practical?
it
provides
the
source
of
catalyse
state the independent variable of this practical and how it is varied
concentration of
hydrogen peroxide
by making a
simple dilution
through mixing with
distilled water
in the correct
proportions
state the controlled variables in the practical
temperature
pH
volume
of
hydrogen peroxide solution
time
allowed to measure
gas volume
length
and
width
of
potato discs
how is the apparatus set up to measure the volume of gas produced?
fill
a
trough
with
water
invert
a
measuring cylinder
and place in the
trough
, and make sure it is
full
of
water
connect the
measuring cylinder
to a
conical flask
with a
delivery tube
why must the bung connecting the conical flask and measuring cylinder be airtight?
so that all the has produced by an
enzymatic reaction displaces
the
water
in the
measuring cylinder
how is rate of reaction calculated?
rate of reaction =
volume
of
gas produced
/
time
state the hazards and safety precautions involved in this practical
hydrogen peroxide
is an
irritant
, wear
safety goggles
and avoid
contact
with
skin
take care when handling the sharp
cork borer
and
scalpel
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