PAG 4: enzyme-controlled reactions

    Cards (9)

    • word equation for the action of a catalase
      hydrogen peroxide -> water + oxygen
    • how is the rate of catalase activity indicated?
      by measuring the volume of oxygen gas released in a fixed time
    • what is the function of potato in this practical?
      it provides the source of catalyse
    • state the independent variable of this practical and how it is varied
      • concentration of hydrogen peroxide
      • by making a simple dilution through mixing with distilled water in the correct proportions
    • state the controlled variables in the practical
      • temperature
      • pH
      • volume of hydrogen peroxide solution
      • time allowed to measure gas volume
      • length and width of potato discs
    • how is the apparatus set up to measure the volume of gas produced?
      1. fill a trough with water
      2. invert a measuring cylinder and place in the trough, and make sure it is full of water
      3. connect the measuring cylinder to a conical flask with a delivery tube
    • why must the bung connecting the conical flask and measuring cylinder be airtight?
      so that all the has produced by an enzymatic reaction displaces the water in the measuring cylinder
    • how is rate of reaction calculated?
      rate of reaction = volume of gas produced / time
    • state the hazards and safety precautions involved in this practical
      • hydrogen peroxide is an irritant, wear safety goggles and avoid contact with skin
      • take care when handling the sharp cork borer and scalpel
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