antibiotic effects

• What is the purpose of aseptic technique?

  To avoid contamination of the sample
• Why is aseptic technique important in experiments?

  It ensures reliable and repeatable data
• What can be measured to determine antibiotic effectiveness?

  Zone of inhibition
• What is used to provide nutrients in the experiment?

  Antibiotic agar plate
• What equipment is needed for the antibiotic effectiveness experiment?

  • Agar plate seeded with bacteria
  • Pipette
  • Bunsen burner
  • Disinfectant
  • Soap
  • Paper towels
  • Forceps
  • Antibiotic impregnated paper discs
  • Sellotape
  • Incubator
• What should be done to surfaces before and after the experiment?

  Wipe down with antibacterial cleaner
• How does a Bunsen burner help in aseptic technique?

  It creates convection currents to draw microbes away
• What should be done to the wire hoop before transferring bacteria?

  Flame the wire hoop
• Why should the neck of bottles be flamed before use?

  To prevent bacteria from entering the vessel
• How long should vessels containing bacteria be kept open?

  For the minimum amount of time
• What should be done to limit air currents during the experiment?

  Close windows and doors
• What are the steps in the method for the antibiotic effectiveness experiment?

  1. Use aseptic technique throughout.
  2. Flame forceps and pick up a paper disc.
  3. Lift lid of petri dish and place disc on agar.
  4. Tape dish with two pieces of sellotape.
  5. Wash hands and disinfect the bench.
  6. Incubate for 24 hours at approximately 30 degrees.
  7. Measure the radius of the clear zone and calculate the area.
• What are the hazards and safety precautions in the experiment?

  • Disinfectant: Flammable; keep away from flames.
  • Naked flame: Fire hazard; keep away from flammable materials.
  • Biohazard: Use aseptic technique and wash hands.
  • Broken glass: Handle carefully; keep away from edges.
• What should be plotted on a bar graph in the experiment?

  Type of antibiotics against area of clear zone
• What does a larger zone of inhibition indicate?

  More effective antibiotics against bacteria
• What factors affect antibiotic effectiveness against bacteria?

  Whether bacteria are gram-positive or gram-negative
• What type of bacteria can affect antibiotic effectiveness?

  Gram-positive or gram-negative bacteria
• What is the relationship between antibiotics and bacteria in the experiment?

  • Antibiotics are tested for effectiveness.
  • Effectiveness is measured by the zone of inhibition.
  • Different antibiotics affect different bacteria types.