Vitamin C content

• What is DCPIP?

  A redox indicator dye
• What color does DCPIP turn when it is reduced?

  Colorless
• What role does Vitamin C play in relation to DCPIP?

  It acts as an antioxidant
• How can DCPIP be used in a practical experiment?

  To measure Vitamin C content in fruit juice
• What equipment is needed for the DCPIP experiment?

  • DCPIP solution
  • Vitamin C solution
  • Fruit juices
  • Test tubes
  • Pipette
• What is the first step in the DCPIP experiment method?

  Transfer 1cm of DCPIP solution into a test tube
• How should Vitamin C solution be added to the DCPIP solution?

  Dropwise, shaking after each drop
• What should be recorded during the DCPIP experiment?

  The volume of Vitamin C used
• What should be done after measuring Vitamin C with DCPIP?

  Repeat the experiment with fruit juices
• What are the hazards and safety precautions associated with DCPIP?

  Hazards:

  • Irritant to skin and eyes
  • May cause staining

  
  

  Safety Precautions:

  • Wear eye protection
  • Keep away from the edge of the desk
• What should be done in case of DCPIP contact with skin or eyes?

  Wash immediately with cold water
• What are the risks associated with broken glass in the lab?

  Risks:

  • Cuts from sharp objects

  
  

  Safety Precautions:

  • Handle glassware carefully
  • Keep glassware away from the edge of the desk
• What should be done if a cut occurs from broken glass?

  Elevate the cut and apply pressure
• What is the risk associated with bags and stools in the lab?

  Risk:

  • Tripping hazards

  
  

  Safety Precautions:

  • Keep bags and stools under desks
  • Clean up any spillages
• What should be plotted on the graph in the experiment?

  Absorbance against ethanol concentration/temperature