Dilute hydrochloric acid to hydrolyse non-reducing sugars
Sodium hydrogencarbonate to neutralise
Heat
Blue -> orange/red
What is a serial dilution?
Transfer 1cm³ of previous solution
Add 9cm³ distilled water
10moldm³ -> 1moldm³ -> 0.1moldm³
What is a condensation reaction?
Joining 2 molecules with a chemical bond by removing water
What is a hydrolysis reaction?
Breaking a chemical bond between 2 molecules by removing water
What do carbohydrates contain?
Only carbon, Hydrogen and Oxygen
What is monosaccharide?
A single unit of a
What are the 3 monosaccharides?
Glucose, fructose and galactose
What is a disaccharide?
Two monosaccharides joined by a glycosidic bond
What is a polysaccharide?
A polymer made of many monosaccharides joined by glycosidic bonds
What are the 3 disaccharides?
Maltose, lactose and sucrose
What is the equation for maltose?
Glucose + glucose -> maltose + water
What is the equation for lactose?
Glucose + galactose -> lactose + water
What is the equation for sucrose?
Glucose + fructose -> sucrose + water
What is the structure of alpha glucose?
C6H12O6
OH groups on the same side (on the bottom)
What is the structure of beta glucose?
C6H12O6
Flipped OH group on carbon 1 (one OH group on the top and one OH group on the bottom)
What are the 3 polysaccharides?
Starch, cellulose and glycogen
What is the structure and function of amylose?
Made of many alpha glucose molecules
Bonded by 1-4 glycosidic bonds
Forms a long, unbranched chain that coils into a helical structure making is good for energy storage
Insoluble in water so does not affect water potential
The helical shape is stabilized by hydrogen bonds
Slower energy release than amylopectin as it has a smaller surface area and ends for enzymes to act on
What is the structure and function of amylopectin?
Made of many alpha glucose molecules
1-4 chains
1-6 branches
Branched structure allows for easier hydrolysis than amylose as it has a higher surface area and ends for enzymes to act on for a faster energy release
Insoluble so does not affect water potential
What is the structure and function of starch?
Made of Alpha glucose
1-4 glycosidic bonds in amylose chains, compact for glucose storage
1-4 and 1-6 in amylopectin branches for a bigger surface area for easier hydrolysis and faster release of energy
Plant cells (e.g, chloroplast)
Made of 2 polymers Amylose- an unbranched helix
Insoluble in water so won't affect water potential
What is the structure and function of cellulose?
Made of Beta glucose
1-4 glycosidic bonds
Structure strength for plant cell wall
Plant only
Polymer forms long straight chains that are held in parallel by many hydrogen bonds to form fibrils
Many hydrogen bonds provide collective strength
Insoluble in water so won't affect water potential
What is the structure and function of Glycogen?
Made of alpha glucose monomers
1-4 amylose chains, helix for glucose storage
Lots of 1-6 amylopectin branches for easier hydrolysis and bigger surface area for faster energy release
Insoluble in water so doesn't affect water potential
Only in animals
More branched than starch for faster energy release