During transcription, a molecule of mRNA is made in the nucleus
The hydrogen bonds between the complementary bases break and the DNA uncoils thus separating the two strands
One of the DNA strands is used as a template by RNA polymerase to make the mRNA molecule. the DNA template is called the antisense strand
Transcription:
3. Free nucleotides line up by complementary base pairing and adjacent nucleotides are joined by phosphodiester bonds made by RNA polymerase thus forming a single stranded molecule of mRNA
4. mRNA then moves out of the nucleus through a pore and attaches to a ribosome in the cytoplasm which is the site of next stage of protein synthesis called translation