formed when two monosaccharides join together by condensation reactions— A glycosidic bond forms between the 2 monosaccharides as a molecule of water is released
Monosaccharides
joined together by condensation reactions, forming a glycosidic bond and releasing a molecule of water
The test sample and iodine dissolved in potassium iodide solution produce
a browny-orange colourindicating no starch
Dark, blue-black colour in the test sample indicates
the presence of starch
Sucrose
is a disaccharide formed from a condensation reaction between : ●glucose ●fructose
Lactose
is a disaccharide formed from glucose + galactose
Maltose
is a disaccharide formed from alpha glucose + alpha glucose
All carbohydrates contain the elements
●C●H●O
Monosaccharides
monomers that carbohydrates are made frome.g : ● Glucose● Fructose● Galactose
Hexose Sugar
a monosaccharide with six carbon atoms in each moleculee.g Glucose
There are 2 types of glucose :
● alpha (a) ● beta (b) glucose — they’re isomers
Isomers
molecules with the same molecular formula as each other, but with the atoms connected in a different way e.g Alpha and Beta Glucose
Glycogen and starch are formed
by the condensation of a-glucose
Cellulose
formed by the condensation of b-glucose
Polysaccharides
are carbohydrates and are formed by the condensation of many glucose units
Biochemical test for starch
using iodine/ potassium iodide
All sugars can be classified as
reducing sugars or non-reducing sugars.
Reducing sugars
● include all monosaccharides● some disaccharides e.g : maltose and lactose.
Reducing sugars can be identified by
● heating a sample with Benedict’s reagent ● observing if a green, yellow, orange, or brick red precipitate forms
The concentration of reducing sugar can be determined by
comparing the colour change of the precipitate in different solutions
To test for sugars
● the Benedict’s test is used● differs depending on the type of sugar being tested for
A positive Benedict's test result
indicated by a brick red colour
A negative Benedict's test result
indicated by a blue colour
To test for non-reducing sugars
e.g sucrose,1. sugars need to be broken down into monosaccharides by getting a new sample of the test solution,2. adding dilute hydrochloric acid, 3. heating it in a water bath that’s been brought to the boil
Benedict's test for non-reducing sugars
● involves heating a sample with Benedict’s reagent● observing if a coloured precipitate forms
Benedict's reagent is
● blue when no reducing sugar present● changes to green, yellow, orange, or brick red when there is
A more accurate way of determining the concentration of reducing sugar is
● filter the solution● weigh the precipitateor ● to remove the precipitate ● use a colorimeter to measure the absorbance of the remaining Benedict’s reagent
Uses of Starch
● Plants use starch as a way of storing excess glucose ● as it is too large to leave cells + insoluble ● Starch can be hydrolysed to release glucose for respiration
Insoluble
doesn’t dissolve in water— this also means it does not affect water potential
Structure of Amylose
● long● unbranched chain of a-glucose ● with angles in the glycosidic bonds ● give it a coiled structure, making it compact for storage
Amylose is good for storage as
● compact● can fit more into a small space
Strucure of Amylopectin
● long● branched chain of a-glucose ( due to 1,6 glcosidic bonds )● with side branches that allow the enzymes that break down the molecule to get at the glycosidic bonds easily● means the glucose can be released quickly
Animals store excess glucose as
Glycogen
Structure of Glycogen
● long● branched chain with lots of side branches (more than amylopectin)● glycosidic bonds are 1,6
Properties of Glycogen
● Lots of branches — increase surface area for enzymes to hydrolyse glycosidic bonds allowing glucose to be released quickly● It is also a compact molecule so it is good for storage
Uses of Glycogen
● Animals store excess glucose as glycogen in muscles and the liver● Glycogen is an energy store — can be hydrolysed to release glucose quickly when needed for respiration ● e.g during exercise
Structure of Cellulose
●major component of plant cell walls●long●unbranched straight chains of b-glucose●1-4glycosidicbonds
Cellulose chains are linked together by hydrogen bonds to
●form strong fibres called microfibrils ●mean cellulose provides structural support for cells ●e.g. in plant cell walls
Properties of Cellulose
●hydrogen bonds between the cellulose chains make the microfibrils very strong ●but still flexible ●allowing them to provide support
Uses of Cellulose
●major structural component in the cell walls of plants ●it provides support ●allows cells to become turgid