IUK 107 Analytical Chemistry

• Qualitative analysis is possible because of matter absorbing light resonating with their energies
• Bond vibration is one of the responses of matter as they interact with light or electromagnetic radiation
• Thermo-effects in spectroscopy deny analysis of organics
• Does a biosynthetic sample contain minerals only?

  • No. Biosynthetic samples contains both organic and inorganic entitles.
• Why does a sample need to be "prepared" for analysis?

  • Sample preparation is required to:
  • separate the organic from inorganics
  • concentrate the analyte by isolation / separation or destruction of the matrix components.
  • reduces background noises and enhances analytic detectability
• What is "analysis"

  A systematic study/investigation aimed at answering one or several questions to solve an issue. Predominancy analysis is preformed by VOKAG, proceed with tool-based classical data acquisition. A scientific analysis is wrapped up by concluding with advance instrumental analysis.
• Name 3 classical analysis.

  • Measure temperature using thermometer.
  • pH measurement using pH meter or litmus paper.
  • Humidity measurement.
• What does the absorption F-T-I-R stand for?

  The absorption F-T-I-R stands for Fourier Transform Infrared - a spectroscopic technique.
• What is that part of an FTIR that makes it superfast?

  Interferometer
• The mathematician who simplified the periodic math concept was:

  Joseph Fourier
• Name the signal - does each signal point up or down?

  T(%) vs wavenumber (sm-1) produces signals pointing down called Troughs
• State the name of the TWO regions that you need to be familiar with

  • Fingerprint region
  • Functional region
• State the wavenumber of the TWO regions that you stated in 5

  • Fingerprint: 500 - 1499 cm-1
  • Functional: 1500 - 3900 cm-1 or

                    1500 - 4000 cm-1
• How about the main signals - hoe many are you expected to be familiar with?

  • OH & NH: 3300 cm-1
  • C=C, C=O & C=N: 1500 - 1900 cm-1
  • C(Triple bond)N, C(Triple bond)C: 2000 - 2500 cm-1
  • C-O, C-H & C-C: 800 - 1300 cm-1
• Is FTIR for Qualitative or Quantitative or Semiquantitative Analysis?

  • Qualitative: Powerful for structural elucidation with a good spectral database. (automated qualitative match procedure)
  • Quantitative: Great with standards database
  • Semiquantitative: Crystallizing estimation - Requires expertise