companion cells have many mitochondria - provides energy from aerobic respiration since pumping sucrose into sieve cells requires active transport + needs energy
comparison between xylem and phloem:
xylem:
physical process
transports water and minerals
transports upwards from roots to leaves
phloem:
requires energy
transports sucrose + amino acids dissolved in water
transports upwards and downwards
transpiration: the process by which water is carried through plants from the roots to the stomata in leaves, where it diffuses out the plant as water vapour and into the atmosphere (physical process)
during transpiration, water moves from root to xylem by osmosis (high to low concentration of water molecules)
threads between cells allow water to pass through
higher pressure at bottom of plant than top forces water up plant (pressure gradient)
hydrogen bonds cause attractive forces between water molecules so unbroken chain of water
concentration of water vapour in air spaces (in leaf) is greater than concentration outside the leaf so water diffuses faster out the stomata
the water being lost from stomata of leaves causes more water to be pulled up through xylem
factors affecting rate of transpiration/wateruptake:
temperature: higher temperature, particles have more kinetic energy, move more, rate increases
humidity: lower humidity, reduces concentration of water outside the leaf, steep concentration gradient, rate increases
lightintensity: high light intensity, pores in stomata open, more water vapour escaping, rate increases
airmovement: high wind speed, removes water vapour from leaf surfaces, rate increases
function of stomata:
control water loss and gas exchange by opening and closing
allow water vapour and oxygen out of the leaf
allow carbon dioxide and oxygen into the leaf
how guard cells work:
plants regulate the size of stomata with guard cells
in bright light the guard cells take in water by osmosis and become turgid, so stomata open
in low light the guard cells lose water and become flaccid, so stomata close
they close in the dark when no carbon dioxide is needed for photosynthesis
they allow gas exchange and control water loss within the leaf.
size of stomata opening controls rate of transpiration
translocation: the movement of sugars and amino acids from the leaves to the rest of the plant
process of translocation:
glucose is produced by photosynthesis in a green leaf/part of a plant
glucose converted to sucrose
sucrose transported in phloem vessels
companion cells actively pump sucrose into sieve tubes
which causes mass flow and REQUIRES ENERGY
companion cells contain mitochondria (site of aerobic respiration)
mitochondria releases energy required to pump sucrose into sieve cells
sucrose converted back to glucose
sucrose is transported from source to sink
source: where glucose is produced
sink: where glucose is used/stored
diagram of a leaf:
how leaves are adapted to photosynthesis + gas exchange:
have large surface area - maximise amount of gases diffused and light absorbed
stomata - allows gases needed to diffuse in & out
waxycuticle - stops water evaporating through epidermis
chlorophyll - green pigment that transfers light energy to chemical
thin - short distance for gases to diffuse into leaf cell
waxy cuticle - reduces rate of water loss
epidermis (transparent) - allow light to pass through to palisade layer
palisade mesophyll - chloroplasts for photosynthesis
spongy mesophyll - air spaces to allow gases to diffuse through leaf (increase efficiency of gas exchange)
guard cells - regulate transpiration + control gas diffusion by controlling open and close of stomata through turgidity and flaccidity
the uptake of water can be measured using a potometer
under normal circumstances, the rate of water uptake gives a measure of the rate of transpiration (they are the same)
a potometer is a piece of capillary tubing to which a plant has been connected
the water uptake is measured by recording the time taken for a bubble in the tube to move a set distance
method of measuring rate of transpiration:
fill reservoir with water
cut leafy shoot diagonally underwater so no air bubbles
insert shoot into rubber stopper at end of capillary tube
set a fan at setting 1
open tap for reservoir so one air bubble in capillary tube is formed
measure distance air bubble has traveled after 1 minute due to water uptake
calculate rate using speed equation
reset bubble by opening tap
repeat on other fan settings
potometer experiment variables:
Independent: speed of fan -> shows how air movement affects rate of transpiration
Dependent: distance travelled by air bubble
Control: plant species, number of leaves, temperature, light intensity, humidity, time