glycosidic bonds= chemical bonds which joins carbohydrates molecules together
quarternary =level of protein structure which multiple polypeptide chains form together to make a protein
non reducing sugars test=following a negative result with a benedicts test, a new sample is mixed with dilute HCL and slowly brought to a boil. this breaks the glycosidic bonds so the solution is neutralised with sodium hydrocarbonate and then tested with benedicts again
primary structure= level of protein structure which shows the sequence of amino acids in a polypeptide
induced fit model=a process where the active site changes its shape after substrate binding to improve affirmation and reduce activation energy.
non competitive inhibitor= type of inhibitor which binds to the secondary binding site, changing the shape of the active site and preventing substrates binding
proteins/amino acids test =biuret mixed with sample at room temp and will change from blue to purple which indicates that proteins is present
competitive inhibitors= bind to active site and prevent substrate binding
activation energy=energy required to start a reaction, enzyme lower the activation energy making the reaction more likely
secondary structure= level of protein structure which shows the effects of hydrogen bonds in a polypeptide which creates alpha helix or beta pleated sheets
peptide bonds=chemical bond which joins amino acids together
ester bonds= hydrogen and carboxyl group, hydrolysis to remove water produces alcohol and carboxylic acid, which joins molecules together to form lipids
hydrolysis= breaking down a chemical bond between two molecules using water as a catalyst
teritory structure = level of protein structure that shows the effect of ionic bonds and disulphide bridges in a polypeptide, this includes only prosthetic(non proteins) groups in a protein
starch = storage for carbohydrates found in plants
formed from alpha glucose
large insoluble molecule so it cant leave the cell
some branches for faster glucose release
monosaccharides= carbohydrate molecules that cannot be broken down into simpler carbohydrates molecules , also called simple sugars e.g. glucose,fructose,galactose
dissaccharides= a carbohydrate containing 2 monosaccharides joined by a glycosidic bond. e.g sucrose, lactose, maltose
triglyceride= a lipid-containing three fatty acids and one glycerol, joined together by an ester bond
function: energy store, thermal indicator, buoyancy, protection of vital organs and water proofing.
cellulose=
carbohydrate which builds plant cell walls
formed by beta glucose
forms long straight chains linked together by hydrogen bonds
provides strength
condensation= water molecules join together to form a hydrogen bond between the oxygen and hydrogen atoms and produces water as a product
glycogen=
storage for carbohydrates found in animal
large insoluble molecules so it cant leave cells
highly branched for rapid glucose release
phospholipid=a lipid consisting of 2 fatty acids, 1 phosphate and 1 glycerol, joined by ester bonds
function: key component of cell membrane
reducing sugars test=
test involves mixing the sample with benedicts solution then heating the solution up
coloured ppt means reducing sugars are present
greater colour change means higher concentration of reducing sugars, not which one is present
emulsion test=shake sample with ethanol, then pour the solution slowly, if the mixture turns milky lipids are present
starch test=at room temp with iodine and solution, iodine will change from orange to blue/black in the presence of starch
high specific heat capacity =water can buffer changes in temperature
strong surface conhesion=water has strong cohesion between molecules allowing water columns of water to form in the xylem and insects to move around on the surface of water
solvent= other chemical can dissolve in water allowing reactions to occur
hydrogen bonding= water molecule attracts another polar molecule by hydrogen bonds forming between them
latent heat of vapourisation = water can provide a cooling effect through evaporation
phosphodiester bond= phosphate group and deoxyribose sugar bond
DNA helicase= breaks hydrogen bonds between DNA strands, untwists DNA strands between complementary base pairs
RNA polymerase= synthesises mRNA using template strand as guide
transcription= process where RNA is made from DNA
ribosomes= site for protein synthesis
messenger RNA (mRNA)= carries genetic information to ribosome
semi conservative replication = DNA replication which produces a double stranded molecule consisting of one original and one newly synthesised strand
restriction enzymes= cut DNA at specific sites
Monomer
Smaller units that join together to form larger molecules