In the section of the graph where the rate is not increasing, the limiting factor will be something other than what is on the x axis, which can be temperature, light intensity, or carbon dioxide concentration.
The method of testing a leaf for starch can also be used to test whether chlorophyll is needed for photosynthesis by using a variegated leaf (one that is partially green and partially white).
The method involves dropping the leaf in boiling water, transferring it into hot ethanol in a boiling tube for 5-10 minutes, rinsing it in cold water, spreading it out on a white tile and covering it with iodine solution.
Starch is stored in chloroplasts where photosynthesis occurs, so testing a leaf for starch is a reliable indicator of which parts of the leaf are photosynthesising.
Photosynthesis provides a source of carbohydrates, but plants contain and require many other types of biological molecules such as proteins, lipids and nucleic acid (DNA).
Although plants synthesise glucose during photosynthesis, their leaves cannot be tested for its presence as the glucose produced is quickly used up, converted into other substances and transported or stored as starch.
Carbohydrates contain the elements carbon, hydrogen and oxygen but proteins, for example, contain nitrogen as well (and certain amino acids contain other elements too).
Two fundamental mineral ions required by plants are nitrogen and magnesium, without a source of these elements, plants cannot photosynthesise or grow properly.