investigating factors affecting enzyme action

•  A tenfold dilution is a logarithmic dilution in which there are a series of dilutions to a factor of ten.
• concentration

  
• PH

  
• temperature

  
• We can calculate rate of enzyme activity by calculating initial rate

  Gradient = change in y-axis / change in x-axis

  
• 1. Set up your data logger and colorimeter using the guide
  2. Label 5 boiling tubes with each pH buffer.
  3. Into each tube add 1cm3 of trypsin and 1cm3 matching pH buffer
  4. Take 5 cuvettes. Place 1cm3 trypsin, 1cm3 pH buffer, 2cm3 of distilled water into each
  5. calibrate the colorimeter using the pH 5 control
  6. Take a new cuvette, add the pH 5 solution with 2cm3 of milk
  7. Place into the colorimeter and start the data logger graph. for 5 minutes
  8. Repeat steps 5 to 7 for each pH buffer, remembering to calibrate the colorimeter
  9. find the initial rates and record these in your table.