Solvents and concentrations effects

• Phospholipids dissolve in organic solvents like methanol and ethanol
• Ethanol causes membrane disintegration as it forms temporary bonds with phospholipid heads , phospholipids move out of place and pigment leaks out
• Ethanol can also effect bonding in proteins and lead to denaturing , further gaps cause more leakage
• Cholesterol is soluble in ethanol this also causes gaps in the membrane and pigment leakage