Microscopy

• The electron microscope uses a beam of electrons to create a highly detailed image of a specimen.
• Magnification = image size/object size
• A compound microscope has two lenses - an objective lens (lower) and an eyepiece lens (upper).
• The light microscope is used to observe cells, tissues, and organisms.
• The fluorescence microscope uses fluorescent dyes to label specific structures within a specimen and create a highly detailed image.
• The compound microscope uses multiple lenses to magnify the image of a specimen.
• Electron microscopes have higher resolution than light microscopes, allowing for the visualization of structures at the nanometer scale.
• There are two types of electron microscopes: transmission electron microscopes (TEMs) and scanning electron microscopes (SEMs).
• Transmission Electron Microscopes (TEM): A type of electron microscope that transmits an electron beam through a thin sample to produce high-resolution images.
• Resolution is the ability to distinguish two closely spaced objects as separate entities, not just one object.
• Objective lens is responsible for magnifying the sample and capturing the image.
• Eyepiece allows you to view the magnified image of the sample
• Stage holds the sample in place for observation
• Condenser focuses the light onto the sample
• Light source provides illumination for the sample
• Coarse adjustment knob used to adjust the focus of the microscope.
• Fine adjustment knob allows for precise focusing of the image
• Arm supports the upper part of the microscope and holds the stage.
• Base provides a stable foundation the microscope