lab contribution

• Gene labelling - use fluorophores or LacZ to track fate driving regeneration to find stem cell's and gene's specific contribution to regeneration
• Lineage Tracking - use Cre/loxP to mark stem cells and their descendants permanently
• Transgenic animals - inducive or constitutive expression of genes associated with the area of study, can modulate expression of MyoD for example to assess its impact
• Cell-specific KO - use CRISPR/Cas9 or conditional KO, investigates importance of genes in specific cell types
• In vivo imaging - allows visualisation of processes such as muscle growth, regeneration and repair
• Myogenesis occurs through proliferative expansion (myoblast fusion) and hyperplasia (increase in myoblast numbers)
• Myogenesis occurs through proliferative expansion (myoblasts) and fusion (myocytes)
• Satellite cells are quiescent until activated during injury or exercise
• Satellite cells are responsible for postnatal skeletal muscle growth and regeneration