Moving Exam

• What method is used in ALT or Serum Glutamic Pyruvic Transferase (SGPT) determination?

  Frankel Reitman (Colorimetric) Method
• What is the Wavelength that Frankel Reitman method use in measuring ALT or SGPT?

  492 nm (490 to 550 nm)
• What is the buffered solution that is used for ALT determination?

  alpha-Ketoglutarate and Alanine
• What analyte is measured in ALT/SGPT in Frankel Reitman method?

  Pyruvic acid
• When pyruvic acid is to be read what is added to it in the final reaction of the method?

  Dinitrophenylhydrazine
• What is the normal range for ALT/SGPT?

  0-30 U/L
• What is the borderline level of ALT?

  31-45 U/L
• What is the level of ALT/SGPT that indicated Post Cardiac Infraction?

  45-100 U/L
• Can hemolysis affect SGPT/ALT results?

  No
• What is the reference range of ALT/SGPT that indicates liver necrosis?

  100-2000 U/L
• What is the method used in AST/SGOT analysis?

  Frankel Reitman Method
• What is the resulting product for the AST/SGOT reaction?

  Oxaloacetic acid
• What is the substrate that's used in AST/SGOT analysis?

  Keto-glutarate and Aspartate
• What is more liver specific enzyme that indicates liver damage between AST and ALT?

  ALT
• What is the normal reference range for AST/SGOT?

  0-40 U/L
• What is the border line level for AST/SGOT?

  41-50 U/L
• What levels of AST/SGOT is associated with post cardiac infraction?

  50-400 U/L
• What levels of AST/SGOT indicates liver necrosis?

  50-1000 U/L
• Within how many days should SGOT be analyzed?

  3-4 days
• What is the incubation time for ALT? And at what temperature?

  15 mins at 37C\
• What is the incubation time for AST? And at what temperature?

  30 mins. at 37C
• For Na+ determination, what method of analysis is used?

  Albanese and Lein
• For K+ determination what is the method of analysis used?

  Lockhead and Purcell
• For Na+ and K+ determination, what is the kind of method used?

  Colorimetric Endpoint
• What is the resulting end color for Na+ determination?

  lavander
• What is the resulting color for K+ determination?

  Blue
• What is the wavelength used in calculating the absorbance of K+?

  600 nm
• What is the wavelength used in calculating the absorbance of Na+?

  550 nm
• What is the normal range of Na+ in the serum?

  134-148 mmol/L
• What is the normal range of K+ in the serum?

  3.6-5.4 mmol/L
• What is the value of standard in the formula for Na+ determination?

  140 mmol/L
• What is the value of the standard in the formula of K+ determination?

  4.5 mmol/L