1.1.5 Microscopy

• What does microscopy refer to?

  Use of microscopes
• What is the maximum magnification of a light microscope?

  2,000x
• What type of samples can be observed under a light microscope?

  Living cells and tissues
• A light microscope uses visible light to magnify objects
• The resolution of a light microscope is higher than that of an electron microscope.

  False
• The electron microscope uses a beam of electrons
• What are two examples of electron microscopes?

  SEM and TEM
• A light microscope uses visible light to magnify objects.
• Samples for electron microscopy must be fixed and dehydrated.


  True
• What is the maximum magnification of a light microscope?

  2,000x
• Light microscopes use visible light to magnify objects.visible light
• What is the maximum magnification achievable by light microscopes?

  2,000x
• What effect does fixing and dehydrating samples have on the natural state of the specimen?

  Alters the natural state
• Steps involved in using a light microscope:

  1️⃣ Prepare the sample

  2️⃣ Place the sample on the stage

  3️⃣ Adjust the focus

  4️⃣ Observe the image
• What type of light do light microscopes use to magnify objects?

  Visible light
• Match the microscope type with its primary use:

  Light Microscope ↔️ Observing living cells

  Electron Microscope ↔️ Studying smaller cellular structures
• Electron microscopes use magnetic lenses to focus the electron beam.


  True
• The magnification capability of a light microscope is up to 2,000x
• What type of samples can be observed using a light microscope?

  Living cells and tissues
• Match the microscope type with its magnification method:

  Light Microscope ↔️ Visible light and lenses

  Electron Microscope ↔️ Beams of electrons
• Light microscopes require extensive sample preparation before observation.

  False
• Which type of microscope can resolve finer details like viruses and macromolecules?

  Electron Microscope
• Light microscopes can observe living cells without altering their natural state.


  True
• Which type of microscope is used in medical diagnostics to identify pathogens?

  Light Microscope
• An electron microscope can resolve structures as small as viruses and molecules.

  True
• Order the steps involved in using a light microscope.

  1️⃣ Prepare the sample

  2️⃣ Place the sample on the stage

  3️⃣ Adjust the focus

  4️⃣ Magnify the image
• What is the maximum magnification of an electron microscope?

  1,000,000x
• An electron microscope uses a beam of electrons to magnify objects.
• What are the two main types of microscopes?

  Light and electron
• Electron microscopes can achieve magnifications up to 1,000,000x.


  True
• Electron microscopes require samples to be fixed and dehydrated.dehydrated
• Match the microscope type with its magnification capability:

  Light Microscope ↔️ Up to 2,000x

  Electron Microscope ↔️ Up to 1,000,000x
• Electron microscopes use a beam of electrons to magnify objects.

  True
• Light microscopes can observe living cells and tissues.living cells
• What are two key subtypes of electron microscopes?

  SEM and TEM
• What type of light does a light microscope use to magnify objects?

  Visible light
• Electron microscopes have lower resolution compared to light microscopes.

  False
• Electron microscopes require samples to be fixed and dehydrated
• What is the resolution capability of a light microscope?

  Lower resolution
• Electron microscopes require samples to be fixed and dehydrated