2.1.3 Microscopy

• Microscopy allows scientists to visualize the internal structure and organization
• The resolution of an electron microscope is 0.1 nm.
• Electron microscopes are suitable for observing living cells.

  False
• Match the components of a compound light microscope with their functions:

  Eyepiece ↔️ Enlarges the image formed by the objective lens

  Objective lens ↔️ Produces a high-resolution magnified image

  Condenser ↔️ Focuses light onto the specimen

  Stage ↔️ Supports and positions the specimen
• What is the primary magnification range of objective lenses in a compound light microscope?

  4x to 100x
• The preparation of slides is crucial for accurate microscopic observation.


  True
• The eyepiece of a compound light microscope typically provides a magnification of 10x
• Which component of a compound light microscope focuses light onto the specimen?

  Condenser lens
• Steps in preparing slides for microscopic examination

  1️⃣ Smearing the sample thinly

  2️⃣ Fixation to preserve cells

  3️⃣ Staining to highlight structures
• What is the purpose of fixation in slide preparation?

  Preserves cell structures
• Microscopy is crucial in cell biology for visualizing cell structures and dynamic processes.


  True
• Match the microscope type with its features:

  Light Microscope ↔️ Low magnification, low resolution

  Electron Microscope ↔️ High magnification, high resolution

  Confocal Microscope ↔️ Produces 3D images, moderate resolution
• An electron microscope uses beams of electrons instead of light to magnify objects.
• Which type of microscope is used to observe mitosis in a tissue sample?

  Light microscope
• What is the typical magnification provided by the eyepiece of a compound light microscope?

  10x
• The coarse adjustment knob is used for initial focusing of the specimen.
• What are the two types of adjustment knobs on a light microscope used for focusing?

  Coarse and fine
• What is the purpose of microscopy in cell biology?

  To study small objects
• Light microscopes have higher magnification and resolution than electron microscopes.

  False
• What is the resolution of a light microscope?

  0.2 μm
• The maximum magnification of a light microscope is 1000x
• What type of microscope has the highest resolution?

  Electron microscope
• Light microscopes use electrons to magnify objects.

  False
• What is a common use case for light microscopes?

  Observing living cells
• What is the maximum magnification of an electron microscope?

  1,000,000x
• The eyepiece of a compound light microscope typically provides a magnification of 10x
• Steps involved in light magnification within a compound light microscope

  1️⃣ Light source illuminates the specimen

  2️⃣ Light is focused by the condenser lens

  3️⃣ Objective lens magnifies the image

  4️⃣ Eyepiece further magnifies the image
• What is the primary function of a compound light microscope?

  To magnify small objects
• Objective lenses in a compound light microscope range from 4x to 100x magnification.

  True
• The stage of a compound light microscope holds the specimen slide
• Smearing a sample ensures cells are evenly distributed for optimal viewing.


  True
• Hematoxylin and eosin (H&E) staining is used to differentiate tissue types
• What are three key applications of microscopy in cell biology?

  Visualize structure, observe processes, identify cell types
• What is the resolution of a light microscope?

  0.2 μm
• A confocal microscope is used for producing 3D cell images.


  True
• Electron microscopes use electron scattering to create images.
• The condenser lens focuses light onto the specimen in a compound light microscope.


  True
• Steps in preparing slides for microscopic examination

  1️⃣ Smearing the sample thinly

  2️⃣ Fixation to preserve cells

  3️⃣ Staining to highlight structures
• Which chemical is commonly used for fixation in slide preparation?

  Ethanol
• Adjusting the diaphragm and condenser lens controls the amount of light reaching the specimen.


  True