Steps involved in tissue culture techniques
1️⃣ Initiation: Prepare a small piece of plant tissue
2️⃣ Callus Formation: Induce callus development on agar
3️⃣ Shoot Formation: Transfer callus to media for shoot growth
4️⃣ Root Formation: Move shoots to media for root development
5️⃣ Acclimatization: Introduce plantlets to outdoor conditions