6.3.2 Polymerase Chain Reaction (PCR)

Cards (22)

  • The DNA template in PCR contains the target DNA sequence to be amplified
  • Arrange the steps of PCR in the correct sequence:
    1️⃣ Denaturation
    2️⃣ Annealing
    3️⃣ Extension
  • The number of target DNA sequences increases exponentially with each PCR cycle.
    True
  • During annealing, primers bind to the target DNA sequence at a temperature between 50-65°C
  • The extension step in PCR occurs at 72°C, with DNA polymerase adding nucleotides to extend the primers.

    True
  • DNA polymerase in PCR extends primers by adding nucleotides to create new DNA strands.

    True
  • The DNA template in PCR provides the target DNA sequence that needs to be amplified
  • What does PCR stand for?
    Polymerase Chain Reaction
  • What are primers in PCR?
    Short DNA sequences
  • PCR involves repeating three main steps in cycles to exponentially increase the number of target DNA sequences.
    True
  • Which enzyme synthesizes new DNA strands in PCR?
    DNA polymerase
  • Match the PCR component with its function:
    DNA polymerase ↔️ Synthesizes new DNA strands
    Deoxynucleotides (dNTPs) ↔️ Building blocks for new DNA strands
  • PCR produces millions of copies of the target DNA sequence by repeating three main steps in cycles.

    True
  • What happens to DNA during the denaturation step of PCR?
    Strands separate
  • PCR stands for Polymerase Chain Reaction
  • Steps involved in a single cycle of PCR
    1️⃣ Denaturation
    2️⃣ Annealing
    3️⃣ Extension
  • The DNA template in PCR contains the target DNA sequence to be amplified
  • Match the PCR component with its description:
    Primers ↔️ Bind to specific DNA regions
    DNA polymerase ↔️ Synthesizes new DNA strands
    Deoxynucleotides (dNTPs) ↔️ Building blocks for new DNA
    DNA template ↔️ Contains target DNA
  • During the denaturation step of PCR, hydrogen bonds between DNA strands break.

    True
  • PCR is used in forensic analysis to amplify DNA from small samples for criminal investigations.

    True
  • Match the PCR application with its description:
    DNA sequencing ↔️ Generates copies for analysis
    Genetic diagnosis ↔️ Detects genetic disorders
    Forensic analysis ↔️ Amplifies DNA from small samples
    Evolutionary studies ↔️ Analyzes ancient DNA
  • Arrange the steps involved in a single PCR cycle in the correct order.
    1️⃣ Denaturation
    2️⃣ Annealing
    3️⃣ Extension