CP9 Chromatography

• The mobile phase in thin-layer chromatography (TLC) is a liquid solvent.


  True
• The two main types of chromatography used in CP9 are paper chromatography and TLC
• The stationary phase in chromatography interacts with and retains the components
• What is the role of the mobile phase in chromatography?

  Carries components through system
• Uniform packing of the stationary phase prevents channeling.


  True
• Match the sample type with its application technique:

  Solid ↔️ Dissolve in solvent and apply

  Liquid ↔️ Direct application
• Components in chromatography separate based on polarity, solubility, and molecular size.

  True
• What is the formula for calculating the Rf value?

  Distance by component / solvent
• By comparing the Rf values to known standards, you can identify the individual substances
• What is the purpose of chromatography?

  To isolate and identify substances
• In paper chromatography, the stationary phase is paper
• What is the mobile phase in gas chromatography (GC)?

  Gas
• In both paper chromatography and TLC, the components separate based on their affinity for the stationary and mobile phases.

  True
• What is the role of the stationary phase in chromatography?

  To interact with components
• The mobile phase in chromatography can be either a liquid or a gas
• Steps for preparing and packing the stationary phase

  1️⃣ Select the stationary phase material

  2️⃣ Prepare the slurry with a solvent

  3️⃣ Pack the column by pouring the slurry

  4️⃣ Level the bed to prevent channeling
• Channeling in the stationary phase can be prevented by ensuring uniform packing.


  True
• What is the purpose of chromatography in fields like biology and forensics?

  To isolate and identify substances
• The mobile phase in paper chromatography is a liquid
• What are the two main types of chromatography used in CP9?

  Paper and TLC
• In paper chromatography, the stationary phase is paper
• The stationary phase in TLC is a thin layer of silica gel.


  True
• How do components separate in chromatography?

  Affinity for phases
• The mobile phase carries the mixture through the stationary phase.


  True
• To prepare the stationary phase, a slurry is created by mixing it with a suitable solvent
• Why is careful application of the mixture important in chromatography?

  Optimizing resolution
• For precise application of the sample, a micropipette should be used to minimize disturbance