3.2.5 Microbiology

• Steps in the essential microbiology techniques:

  1️⃣ Aseptic technique

  2️⃣ Inoculation

  3️⃣ Incubation

  4️⃣ Observation
• Mastering essential microbiology techniques allows for effective study of microorganisms.

  True
• What is microbiology the study of?

  Microscopic organisms
• Match the microorganism with its key feature:

  Bacteria ↔️ Prokaryotic cells

  Fungi ↔️ Eukaryotic cells

  Viruses ↔️ Require host cells
• Viruses contain a protein coat and genetic material.

  True
• Bacteria are prokaryotic cells that multiply via binary fission
• What is the term for the asexual reproduction of bacteria?

  Binary fission
• What is the purpose of investigating bacterial growth using agar plates?

  Observe bacterial colonies
• Match the technique with its purpose:

  Inoculation ↔️ Transfer microbes onto media

  Incubation ↔️ Provide optimal growth

  Observation ↔️ Examine microbial growth
• Bacteria are prokaryotic cells that possess a cell wall
• In microbiology practicals, students observe the growth of bacterial colonies
• Students compare the growth of fungal colonies
• Match the microbiology technique with its key steps:

  Aseptic Technique ↔️ Sterilize equipment, clean workspace, hand hygiene

  Inoculation ↔️ Transfer microbes to media, use sterile tools

  Incubation ↔️ Maintain ideal temperature, humidity

  Observation ↔️ Examine growth, note features
• Proper hand hygiene is a key step in maintaining aseptic technique.


  True
• Bacteria reproduce sexually.

  False
• What type of genetic material do viruses contain?

  Genetic material
• Viruses are considered living cells.

  False
• Match the microorganism with its key feature:

  Bacteria ↔️ Prokaryotic cells

  Fungi ↔️ Eukaryotic cells

  Viruses ↔️ Genetic material in protein coat
• In investigating the growth of fungi using agar plates, students inoculate the plates with different fungal
• Steps in aseptic technique:

  1️⃣ Sterilize equipment and work area

  2️⃣ Maintain clean workspace

  3️⃣ Practice proper hand hygiene
• What are some features to note when observing microbial colonies?

  Size, color, texture
• Steps in proper disposal of contaminated materials:

  1️⃣ Use designated biohazard containers

  2️⃣ Follow disposal protocols

  3️⃣ Disinfect surfaces and equipment
• Bacteria are prokaryotic cells that multiply via binary fission.


  True
• Inoculated media are placed in an incubator
• Microbial growth is typically documented using images or drawings.


  True
• Gram staining can differentiate bacteria based on their cell wall composition.


  True
• What is the focus of microbiology as a field of study?

  Microscopic organisms
• Viruses are cells.

  False
• Fungi possess hyphae and reproduce sexually or asexually
• The effectiveness of antiseptics is tested by measuring the inhibition of microbial growth
• Why is aseptic technique essential in microbiology?

  Prevent contamination
• What type of genetic material is found in viruses?

  DNA or RNA
• Students test the effectiveness of antiseptics against bacteria and fungi in microbiology practicals.


  True
• What is the purpose of aseptic technique in microbiology?

  Prevent contamination
• Why is aseptic technique critical in microbiology experiments?

  Prevents contamination
• What does PPE stand for in microbiology safety precautions?

  Personal Protective Equipment
• What is microbiology the study of?

  Microscopic organisms
• What is the process by which prokaryotic cells like bacteria multiply?

  Binary fission
• What is the study of microscopic organisms called?

  Microbiology
• Steps in investigating the growth of bacteria using agar plates:

  1️⃣ Inoculate agar plates with bacterial samples

  2️⃣ Observe and compare bacterial colonies