Cards (57)

    • Gene insertion involves placing an isolated gene into another organism's DNA.

      True
    • What does recombinant DNA technology involve?
      Cutting and splicing DNA
    • What is the purpose of DNA sequencing?
      Determining nucleotide order
    • What is the purpose of recombinant DNA technology?
      Creating new genetic sequences
    • DNA sequencing determines the exact order of nucleotides
    • Match the genetic engineering technique with its description:
      Recombinant DNA technology ↔️ Cutting and splicing DNA segments
      Polymerase Chain Reaction (PCR) ↔️ Amplifying specific DNA sequences
      Gel electrophoresis ↔️ Separating DNA fragments by size
      DNA sequencing ↔️ Determining the order of nucleotides
    • Order the steps involved in creating recombinant DNA:
      1️⃣ Isolate the desired gene
      2️⃣ Cut the gene and plasmid DNA
      3️⃣ Combine the gene and plasmid
      4️⃣ Insert the recombinant DNA into a host
    • What does gel electrophoresis separate DNA fragments based on?
      Size and charge
    • Steps in Recombinant DNA technology
      1️⃣ Identify a desired gene from one organism
      2️⃣ Use enzymes to cut the DNA and extract the gene
      3️⃣ Insert the gene into the DNA of another organism
    • What process is repeated during PCR to make copies of the target DNA?
      Heating and cooling
    • Steps in Gene cloning
      1️⃣ Isolate a target gene from an organism's DNA
      2️⃣ Insert the gene into a vector (e.g. plasmid)
      3️⃣ Introduce the vector into a host organism
    • Gel electrophoresis is a simple and cost-effective method for analyzing DNA fragments
    • DNA sequencing determines the genetic composition of a DNA molecule.

      True
    • The Polymerase Chain Reaction (PCR) requires DNA primers, DNA polymerase enzyme, and nucleotides
    • What type of sample is required for PCR?
      Small DNA sample
    • Recombinant DNA technology involves combining DNA from different organisms.

      True
    • Steps in gene cloning
      1️⃣ Isolate a target gene from an organism's DNA
      2️⃣ Insert the gene into a vector
      3️⃣ Introduce the vector into a host organism
    • PCR is used for the rapid detection and diagnosis of genetic diseases.
    • What is an example of a therapeutic protein produced using gene cloning?
      Insulin
    • What is the definition of genetic engineering?
      Altering an organism's genes
    • What happens to bacteria after the insulin gene is inserted into their DNA?
      They produce insulin
    • Polymerase Chain Reaction (PCR) allows the rapid amplification of specific DNA sequences
    • Gene cloning creates multiple identical copies of a gene within a host organism
    • PCR amplifies specific DNA sequences to produce a large number of copies.

      True
    • Genetic engineering allows scientists to introduce new or enhanced characteristics in organisms.

      True
    • What does recombinant DNA technology create by combining genetic material from different sources?
      New genetic sequences
    • What is recombinant DNA technology used for?
      Creating new genetic sequences
    • DNA sequencing determines the exact order of nucleotides in DNA.

      True
    • Recombinant DNA technology creates novel genetic combinations.

      True
    • In gel electrophoresis, DNA samples are loaded into a gel matrix.

      True
    • What enzymes are required in recombinant DNA technology to cut and splice DNA?
      Restriction enzymes
    • What is the purpose of genetic engineering?
      Altering an organism's genes
    • Steps in PCR
      1️⃣ Obtain a small sample of DNA
      2️⃣ Add DNA primers, DNA polymerase, and nucleotides
      3️⃣ Repeatedly heat and cool the sample
    • Steps in recombinant DNA technology
      1️⃣ Identify a desired gene from one organism
      2️⃣ Use enzymes to cut the DNA and extract the gene
      3️⃣ Insert the gene into the DNA of another organism
    • In gel electrophoresis, DNA samples are loaded into a gel
    • What is the first step in gene cloning?
      Isolate a target gene
    • Match the technique with its key difference:
      Recombinant DNA technology ↔️ Combines DNA from different sources
      Polymerase Chain Reaction (PCR) ↔️ Amplifies specific DNA sequences
      Gel electrophoresis ↔️ Separates DNA by size
      DNA sequencing ↔️ Determines nucleotide order
      Gene cloning ↔️ Creates multiple copies of a gene
    • What is one application of gel electrophoresis?
      Identify genetic mutations
    • One example of gene isolation is selecting a gene for insulin production
    • Match the step in genetic engineering with its example:
      Gene isolation ↔️ Selecting a gene for insulin production
      Gene insertion ↔️ Inserting the insulin gene into bacteria
      Modification ↔️ Bacteria producing insulin
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