1.1.2 Microscopy

• Light microscopes use a beam of electrons to magnify specimens.

  False
• Electron microscopes have a resolution down to 0.1 nanometers
• Match the microscope type with its key feature:

  Light Microscope ↔️ Uses visible light

  Electron Microscope ↔️ Uses a beam of electrons
• Electron microscopes are much more expensive than light microscopes
• Light microscopes can achieve magnification up to 1000x
• Light microscopes can achieve magnification up to 1000x
• To calculate total magnification, multiply the objective lens magnification by the eyepiece lens magnification
• There are two main types of microscopes used in biology: light microscopes and electron microscopes.


  True
• Electron microscopes can achieve a higher magnification than light microscopes.


  True
• What is the formula for calculating total magnification using a light microscope?

  Objective × Eyepiece
• Avoid touching the lenses of a microscope to prevent fingerprints and smudges
• Use lens paper and lens cleaning solution to clean the lenses
• Store the microscope in a dry, cool place to prevent damage
• What are the four key techniques in sample preparation for microscopy?

  Staining, fixing, sectioning, mounting
• Steps in the sample preparation technique of fixing

  1️⃣ Immerse in fixative

  2️⃣ Wash

  3️⃣ Store
• Proper sample preparation ensures high-quality microscopy results.


  True
• Electron microscopes are less expensive than light microscopes.

  False
• There are two main types of microscopes used in biology: light microscopes and electron microscopes
• Electron microscopes use visible light to magnify specimens.

  False
• Electron microscopes are very expensive
• Light microscopes have better resolution than electron microscopes.

  False
• Light microscopes have a resolution of 0.1 nanometers.

  False
• Electron microscopes have a resolution down to 0.1 nanometers
• What is the maximum magnification of a light microscope?

  1000x
• If an objective lens has a magnification of 40x and the eyepiece lens has a magnification of 10x, what is the total magnification?

  400x
• What does an electron microscope use instead of light to magnify specimens?

  Electrons
• Steps to calculate total magnification using a light microscope:

  1️⃣ Identify the objective lens magnification

  2️⃣ Identify the eyepiece lens magnification

  3️⃣ Multiply the objective lens magnification by the eyepiece lens magnification
• How should a microscope be carried to ensure safety?

  With both hands
• What type of cloth should be used to clean the exterior of a microscope?

  Soft, lint-free cloth
• Why should a microscope be kept covered when not in use?

  To protect from dust
• A dim image in a microscope could be caused by a burnt-out bulb.


  True
• Formaldehyde is a common fixative used in sample preparation.


  True
• Mounting uses media like glycerol or resin to preserve the sample
• Electron microscopes use a beam of electrons instead of light.
• Electron microscopes are essential for studying intricate cellular details like ribosomes
• Light microscopes have a magnification of up to 1000x
• What is a major advantage of electron microscopes over light microscopes?

  Higher magnification
• What is the maximum magnification of an electron microscope?

  1,000,000x
• Arrange the following features to describe the differences between magnification and resolution:

  1️⃣ Magnification: How much larger an image appears

  2️⃣ Resolution: Ability to distinguish two separate points

  3️⃣ Magnification: Enlarges the image size

  4️⃣ Resolution: Provides image clarity
• Match the microscope feature with its correct type:

  Magnification up to 1,000,000x ↔️ Electron Microscope

  Resolution up to 0.2 micrometers ↔️ Light Microscope