1.1.4 Microscopy

• There are two main types of microscopes: light and electron microscopes.


  True
• Electron microscopes can achieve magnification up to 1,000,000x
• What is the function of the eyepiece in a compound light microscope?

  Final magnification
• The objective lens of a compound light microscope forms the primary image
• The condenser lens focuses light from the illuminator onto the specimen
• Steps for using a compound light microscope

  1️⃣ Prepare the specimen by placing the slide on the stage

  2️⃣ Adjust the illumination using the illuminator and condenser lens

  3️⃣ Start with the lowest magnification objective lens

  4️⃣ Focus the image using the coarse and fine adjustment knobs

  5️⃣ Increase magnification as needed
• Match the microscope type with its illumination source:

  Light Microscope ↔️ Visible light

  Electron Microscope ↔️ Electron beam
• Light microscopes typically use visible light
• Light microscopes can achieve magnifications up to 1,000,000x.

  False
• Order the steps in how the components of a compound light microscope work together:

  1️⃣ Illuminator provides light

  2️⃣ Condenser lens focuses light on the specimen

  3️⃣ Objective lens magnifies the image

  4️⃣ Eyepiece further magnifies the image
• The objective lens forms the primary image
• Steps involved in using a compound light microscope

  1️⃣ Prepare the specimen

  2️⃣ Adjust the illumination

  3️⃣ Start with the lowest magnification

  4️⃣ Focus the image

  5️⃣ Increase magnification

  6️⃣ Adjust the focus again
• What is the formula for calculating total magnification of a microscope?

  Eyepiece × Objective
• Steps involved in preparing slides for microscope observation

  1️⃣ Obtain a clean glass slide

  2️⃣ Place the specimen on the slide

  3️⃣ Add a coverslip

  4️⃣ Secure the coverslip
• Match the type of microscope with its characteristics:

  Light Microscope ↔️ Visible light, simple preparation

  Electron Microscope ↔️ Electron beam, complex preparation
• The illuminator provides the light source for viewing the specimen.

  True
• The objective lens of a compound light microscope provides the initial magnification
• What is the purpose of the stage in a compound light microscope?

  Holds the specimen slide
• Which knobs are used to focus the image in a compound light microscope?

  Coarse and fine adjustment
• Using a compound light microscope begins with proper preparation of the specimen
• After preparing the specimen, adjust the condenser lens to focus the light
• How is total magnification calculated in a compound light microscope?

  Eyepiece x objective
• What is the primary function of the nucleus in a cell?

  Controls cell's activities
• Why is light microscopy widely used in biology?

  Simplicity and living cell observation
• What type of beam do electron microscopes use for illumination?

  Electron beam
• What is a key feature of light microscopes compared to electron microscopes?

  Cheaper and easier to use
• The condenser lens focuses light from the illuminator onto the specimen
• The revolving nosepiece allows the user to switch between different objective lenses.


  True
• Which component of a compound light microscope provides the light source?

  Illuminator
• Why should you avoid touching the objective lens of a compound light microscope?

  To prevent damage
• What is the maximum magnification of a light microscope?

  2,000x
• Electron microscopes use an electron beam for illumination.


  True
• The resolution of a light microscope is around 0.2 micrometers
• Which lens provides the final magnification in a compound light microscope?

  Eyepiece
• The revolving nosepiece allows the user to switch between different objective lenses.


  True
• To view a specimen in more detail, the user should rotate the nosepiece to a higher magnification objective
• If an eyepiece has a magnification of 10x and the objective lens has a magnification of 40x, the total magnification is 400x
• Adding a coverslip helps avoid air bubbles in the specimen.


  True
• The electron microscope has a magnification of up to 1,000,000x
• Steps involved in the light path through a compound light microscope

  1️⃣ Illuminator provides light

  2️⃣ Condenser lens focuses light

  3️⃣ Objective lens magnifies

  4️⃣ Eyepiece further magnifies