Enzyme experiment

Created by

Dorothy Obi

Cards (6)

Test for PH
1. Add 3 drops of washingup liquid.
2. Add 10cm3 of specific pH buffer (4,6,9).
3. Place in water bath at 25 degrees.
4. Add 10 cm3 of H2O2.
5. Add 10cm3 of Catalase (Celery) into a graduated cylinder. 6. Note how much foam is formed. pH 9 is the optimum pH.
To Test for Temperature
1. Add 3 drops of washingup liquid.
2. Add 10cm3 of pH9 buffer.
3. Add 10 cm3 of H2O2
4. Place in water bath at 0, 25 and 60 degrees.
5. Place 10 cm3 Catalase into each water bath also.
6. Note how much foam is formed at each temperature.
25 degrees is the optimum temperature
To Immobilise Enzymes
Materials needed
Yeast
Sodium Alginate
Calcium chloride
Sucrose
To Immoblise Enzymes (1)
Mix sodium alginate and water and yeast in a beaker.
Draw the mixture into a syringe.
Release the mixture from the syringe, one drop at a time, into the calcium chloride solution. Beads containing yeast cells will form.
Leave the beads to harden.
Filter the beads through a sieve and rinse with distilled water.
To Immoblise Enzymes (2)(application)
6. Mix yeast and water and pour into a separating funnel (Free yeast).
7. Place the beads into another separating funnel (Immobilised yeast).
8. Pour sucrose solution into each of the separating funnels.
9. Using Benedict's solution, immediately test samples from each funnel for glucose. (should change from blue to red)
10. Repeat the test at intervals until glucose appears in both
11.Compare the Clarity of the solutions from both funnels.
12.Record Result (immoblised will be slow but clear whereas free yeast is quick and cloudy)
Denaturing Experiment
1.Add the heated yeast and the buffer to the graduated cylinder.
2.Add hydrogen peroxide to a boiling tube.
3.Stand the cylinder and boiling tube in the water bath until the desired temperature (250C) is reached.
4.Add the hydrogen peroxide into the cylinder.
5.Note the presence or absence of foam formation and record. 6.Repeat the procedure using an unheated radish sample.