Cell culture

• Bacteria multiply by binary fission.
• Bacteria can be grown in a nutrient broth solution or as colonies on an agar gel plate.
• It is important to sterilise solutions and equipment to kill the bacteria already on them
• The inoculating loop is sterilized to incinerate any contaminants.
• the lid of the Petri dish should be secured with adhesive tape to prevent microorganisms from the air contaminating the culture, and stored upside down to stop condensation drops falling onto the agar surface.
• cultures should be incubated in school laboratories at 25 degrees to prevent the growth of harmful pathogens.
• to calculate the area of a circle, use πr²
• we dip the inoculation loop into the microorganism solution and make streaks on the surface of the agar plate which allows the bacteria to spread out and to grow in individual colonies on the agar plate.