Santiago Ramon y Cajal and Camillo Golgi
In the early 20th century, Santiago Ramón y Cajal and Camillo Golgi began working on the structure of the neuron.
Golgi developed a silver staining method that could entirely stain several cells on a particular area, leading him to believe that neurons were directly connected with each other in one cytoplasm.
Cajal challenged this view after staining areas of the brain that had less myelin and discovering that neurons were discrete cells.
Cajal also discovered that cells transmit electrical signals down the neuron in one direction only.