How would you test an enzymes (amylase) optimum pH?
1. Place drops of iodine onto dropping tile
2. Mix starch solution with pH buffer of pH testing
3. Mix with amylase and start timer.
4. Every 10s remove a drop of soltion and add to iodine drop. Continue till blue/black colour is no longer seen
5. Change pH buffer in step 2 and repeat
6. Optimum pH is the one which see's all the starch broken down quickest (shortest time for it to stop turning blue/black)