microscopy

• What is the base of a light microscope used for?

  Supporting the microscope
• The arm of a light microscope connects the base to the top
• The light source in all microscopes is a lamp.

  False
• What is the purpose of the light source in a light microscope?

  Reflect light
• Where is the microscope slide placed on a light microscope?

  The stage
• Objective lenses in a light microscope have different magnifications
• The eyepiece lens of a light microscope has a fixed magnification.
• The coarse and fine focusing knobs are used to get the image in focus
• What is the body tube of a light microscope?

  Connects lenses
• The image seen through a microscope is called the object.

  False
• What does the term "object" refer to in microscopy?

  The real sample
• What is the image in microscopy?

  What you see
• How light travels through a light microscope

  1️⃣ Light from the room hits the mirror

  2️⃣ Light is reflected upwards through the object

  3️⃣ Light passes through an objective lens

  4️⃣ Light passes through the eyepiece lens

  5️⃣ Light enters the eye
• Magnification is defined as how many times larger the image is than the object
• What is the purpose of lenses in a light microscope?

  Spread light rays
• What is the equation for magnification?

  $Magnification =$$\frac{Image\:size}{Object\:size}$
• Resolution is the shortest distance between two points on an object that can still be distinguished as two separate entities
• If an image appears 500 times larger than the object, the magnification is ×500.
• What does high resolution mean in microscopy?

  Detailed and clear image
• Lower resolution images appear blurrier than higher resolution images.
• Light microscopes are small, easy to use, and relatively cheap
• What is the most common type of microscope used in classrooms?

  Light microscope
• What type of radiation do light microscopes use?

  Light
• The resolution of light microscopes is limited by the wavelength of light.
• What is the resolution of light microscopes?

  0.2 micrometers
• Light microscopes are suitable for studying individual cells
• Electron microscopes are portable and easy to use in classrooms.

  False
• Why are light microscopes not suitable for studying subcellular structures like mitochondria?

  Low resolution
• What do electron microscopes use instead of light?

  Electrons
• The wavelength of electrons in electron microscopes is 0.1 nanometers
• What is the maximum resolution of electron microscopes?

  0.1 nanometers
• A smaller resolution value indicates better image clarity.
• Electron microscopes have 2,000 times better resolution than light microscopes
• Which type of microscope allows for higher magnification without blurring?

  Electron microscope
• Electron microscopes can be used to study subcellular structures like mitochondria.
• Match the microscope type with its resolution:

  Light microscope ↔️ 0.2 micrometers

  Electron microscope ↔️ 0.1 nanometers
• Light microscopes are more expensive than electron microscopes.

  False
• Electron microscopes use electrons instead of light
• What is the central structure visible in the image of a pancreatic cell taken with an electron microscope?

  Nucleus
• Order the steps to compare light and electron microscopes:

  1️⃣ Identify the key features of light microscopes

  2️⃣ Identify the key features of electron microscopes

  3️⃣ Compare their resolution

  4️⃣ Compare their advantages