cut onion and separate into layers - tweezers to peel off epidermal tissue from bottom of layers
with tweezers place, epidermal tissue into water on slide
add a drop of iodine solution
place a cover slip on top - stand cover slip upright on slide next to water, carefully tilt and lower it so it covers the specimen. Try not to get any air bubbles under that obstruct your view
Use light microscope to look at slide:
clip slide onto stage
Select lowestpoweredobjectivelens
Use course, adjustmentknob to move the stage up to below the objectivelens
Look down the piece use the causeadjustmentknob to move the stage downwards until images in focus
Just focus with finaladjustmentknob until we get a clear image
if needed greatermagnification, swap to high poweredobjective,lens and refocus
Label microscope
Draw microscope observations: draw the image of the object you are observing, label important features and include title and magnification