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MICB Exam 1
MICB Lecture 5
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Cards (17)
Family, molecular, how long
circular, single stranded
RNA
molecule
family:
pospiviroidae
(replicate in nucleus)
250-430
nucleotides long
Which is which
PrPsc differs from PrPc in its 3
dimensional
conformation, it is
stable
and
aggregates
with other PrPsc
A)
PrPc
B)
PrPsc
2
eukaryotic microbes exhibit both
sexual
and
asexual
reproduction
Prokaryotic microbes reproduce
asexually
through
binary fission
FtsZ
a key
bacterial cytoskeletal
protein in bacteria and archaea
FtsZ features
Homologue of eukaryotic protein tubulin
required for
cell division
and
septum formation
forms
contractile ring
at
midcell
energy from
hydrolysis
of
GTP
batch culture
closed vessel (
test tube
or
flask
),
single
batch of
medium
,
population growth
can be observed when microbes are cultivated in this
What is this
Bacterial
growth
curve
A)
lag phase
B)
exponential phase
C)
stationary phase
D)
death phase
4
lag phase
period following intro of microorganisms into fresh culture
no growth
- cells
synthesizing
new
components
can
differ
exponential phase
balanced,
constant
growth
2, 4, 8, 16, 32
growth expressed as
generation
(doubling time): time required for cells to
divide
stationary phase
Population growth
ceases
due to
carrier capacity
lack of
oxygen
toxic
biproducts
Death phase
nutrient deprivation
and
buildup
of
toxic wastes
cause
irreparable harm
to cells
Spores
stress resistant,
dormant
cells
forms when
mother
cell
lysis
A)
bacillus
1
Name/disease
Clostridium tetani
causes
tetanus
, spores form at
one
pole
direct cell counts
measures microbial growth
Petroff-Hausser method of counting
chambers
drawback:
overestimate
cell count
Viable cell counts - measuring microbial growth
Plating
- colony forming units, combine with
turbidity
measurements
turbidity measurements - measuring microbial growth
microbial cells
scatter
when
light
strikes them
more
turbid
= more
cells
= more
light scattered
=
higher
absorbance reading on
spectrophotometer