Photosynthesis is the process by which plants make glucose from sunlight, an endothermic reaction in which energy is transferred from the environment to the chloroplasts by light.
With an increase in temperature, the rate of photosynthesis increases, as the reaction is controlled by enzymes, this trend continues up to a certain temperature until the enzymes begin to denature and the rate of reaction decreases.
Chlorophyll is a pigment in the leaf that converts light energy to food for the plant, and is therefore essential, if chlorophyll levels are reduced through a magnesium deficiency, then the rate of photosynthesis would decrease.
The rate of photosynthesis can be measured by carrying out an experiment measuring the oxygen production of a plant, this involves placing pondweed in a test tube full with water, sealing the top with a bung, attaching a capillary tube containing water to a syringe, placing a lamp at a measured distance from the test tube, and measuring the distance the bubble has moved to calculate the volume of oxygen produced.
If there is not enough oxygen being supplied, anaerobic respiration takes place instead, with lactic acid as a byproduct, this reaction builds up creating an oxygen debt as oxygen is needed to break lactic acid down.
Farmers can use the knowledge of limiting factors to enhance the conditions in the greenhouse for a greater rate of photosynthesis, leading to increased growth and profits.
Metabolism is the sum of all the reactions in a cell or the body, the energy transferred in the cells by respiration is used in the processes of metabolism to make new molecules.
Inverse proportion describes a relationship between two factors which involves one increasing whilst one decreasing, as the distance between the light source and the plant increases, the light intensity decreases.
Examples of metabolic reactions include glucose molecules being converted to starch, glycogen and cellulose by being joined together, a glycerol molecule and three molecules of fatty acids forming a lipid molecule, glucose and nitrate ions forming amino acids, which are used to form proteins, and the reactions in respiration.
Glucose from photosynthesis is used for respiration, converted into insoluble starch for storage in roots, stems and leaves, used to produce fat or oil for storage in seeds, used to produce cellulose to strengthen cell walls, and combined with nitrates (absorbed from the soil) to form amino acids which produce proteins.
During exercise, more energy is needed for the muscles to contract, meaning respiration has to occur faster, and more oxygen needs to be supplied to cells (and more CO2 removed).
In plant and yeast cells, the oxidation of glucose is incomplete, with glucose converting to ethanol and carbon dioxide, this reaction is used to make bread and alcoholic drinks.
Anaerobic respiration does not use oxygen and yields less energy, it is only used as a last resort, for example during a sprint where it is difficult to breathe in enough oxygen.
Many variables can be changed to observe their effect on photosynthesis: temperature, time, light intensity, carbon dioxide concentration, and the amount of chlorophyll.
A graph involves one limiting factor if it has one line which levels off, with the factor on the horizontal axis and rate of photosynthesis on the vertical axis.
A graph with two lines represents two limiting factors in two experiments, the investigation involves increasing the factor on the horizontal axis, and is carried out at two different other environmental conditions, such as two different temperatures.