When working with samples of human populations, studies must take account of possible variations owing to differences between the sexes resulting from people of different ages taking part in people's lifestyles, which could affect the study.
In scientific studies, it may be difficult to control lifestyle factors that have the potential to affect the study, particularly if the study is long-term.
People involved in a long-term study of the effects of alcohol intake, for instance, will have different lifestyle factors, for instance diet and exercise, that could potentially affect the results of the study.