microscopy

• Magnification - the ratio of the apparent size of an object as seen through the microscope and the actual size of the object
• Resolution/ resolving power - the ability of the lens toclearly separate and distinguish two points of two lines individually in the image
• resolving power - it determined by the shortest wavelength of visible light and maximum numerical aperture
• Numerical aperture - a measurement of the ability of the condenser and the objective lens to gather light.
• Numerical aperture - magnification a numerical aperture
• Focal length - thickness of the object that maybe seen at one time under focus.
• Focal length - 1/a numerical aperture
• Working distance - distance between the front lens of the objective lens and the top of cover glass when the specimen is in focus
• Working distance - 1/a magnification
• Parfocal - refers to the quality of the objective and eyepiece where practically no change in focus has to be made when objective is substituted for another
• Refractive index - Bending of light rays away from the objective lens when light passes from the glass of the microscope slide to the air