W1LECHEMA2

• Factor XIII

  Fibrin Stabilizing factor
• Factor XII

  Hageman factor
• Factor XI

  Plasma Thromboplastin Antecedent (PTA)
• Factor X

  Stuart-Prower factor
• Factor IX


  Christmas factor or Plasma Thromboplastin Component (PTC)
• Factor VIII

  Antihemophilic factor (AHF)
• Factor VII

  Stable Factor
• Factor V

  Labile Factor
• Factor IV

  Calcium Ions
• Factor III

  Tissue Thromboplastin
• Factor II

  Prothrombin
• Factor I

  Fibrinogen
• ATP

  ADP

  Calcium

  Magnesium

  Pyrophosphate

  Serotonin

  Dense granules
• 1). Beta thromboglobulin

  2). Platelet factor 4

  3). FIbrinogen, Fibrionectin

  4). Factor V

  5). PDGF

  6). HMWK, Factor VIII: vWF

  7). Thrombospondin

  8). Plasminogen

  9). Alpha-1-antiplasmin

  10). C1 esterase inhibitor

  Alpha granules
• Organelle zone

  Metabolic center—regulates metabolic activities—aids in platelet activation. Has mitochondria and storage granules. Granules are impt.

  • alpha-most

  • dense granules- some

  lambda—few

  
  

  Granules release their contents with platelet activation

  Activation—energy dependent process.

  
  

  Platelets are full of glycogen. When fully activated, involves oxidative phosphorylation—gets from mitochondria

  
  

  • when platelets are resting-don't use mitochondria—use glycolysis and oxidative Krebs cycle

  
  

  • when active—use oxidative phosphorylation—get a ton more energy
• Thrombostenin (actomyosin)


  major contractile protein

  
  

  - composed of actin and myosin

  - 15% of total proteins in platelets
• Microfilaments

  contractile process (changing of shape), 50nm
• Microtubules


  contributes to thediscoid shapeof an inactivated platelet
• Sol-gel zone


  stable gel component to regulate the arrangement of the internal organelles

  
  

  - microtubules

  - microfilaments

  - thrombostenin (actomyosin)
• Submembrane Area

  prevent contact between organelles and cell membrane

  
  

  - sub membrane filaments

  - base for pseudopods

  - interact with contractile proteins
• Open Canular System (OCS)


  akaconnected canalicular system

  
  

  - exchange of substance in and out of the platelet
• Factor VIII (VIII:von williebrand factor:vWF)

  blood coagulation and platelet adhesion
• Plasma membrane


  physical and chemical barrier between intra and extracellular environment

  
  

  - phospholipids, phosphatidylserine, phophatidylcholine, phosphatidyl inositol

  
  

  - Factor VIII
• I, V, VIII, X, XI, XII, XIII


  Surface for adherance of coagulation factors
• Ia, Ib, Ic, IIa, IIb, III, IV, & V


  contains glycoproteins required forplatelet adhesion and aggregation
• Glycocalyx

  The external surface of a plasma membrane that is important for cell-to-cell communication

  
  

  thickness: 10 to 50um
• Peripheral zone

  glycocalyx

  plasma membrane

  Open Canular System (OCS)

  Submembrane area
• Platelet structure

  peripheral zone

  sol-gel zone

  organelle zone
• None

  Nucleus of platelets
• light blue to purple

  very granularCytoplasm of platelets
• 1-4um


  size of platelets
• Numerous aggregated granules

  Granules of Mature Megakaryocyte
• 8 or more (up to 32)

  Nucleus of Mature Megakaryocyte
• presence of coarse clumps of aggregated granules

  Cytoplasm of Mature Megakaryocyte
• < 1:1


  N/C ratio of Mature Megakaryocyte
• 40-120um

  Size of Mature Megakaryocyte
• very fine


  , numerous granulesGranules of Granular Megakaryocyte
• 4 or more; multi-lobed: small in comparison to cell size

  Nucleus of Granular Megakaryocyte
• abundant,

  pinkish blueCytoplasm of Granular Megakaryocyte
• 2:1


  N/C ratio of Granular Megakaryocyte