Microscope Unit

• What color is the 4X objective lens?

  red
• What color is the 10X objective lens?

  yellow
• What color is the 40X objective lens?

  blue
• What color is the 100X objective lens?

  white
• What stain was used for onion cells?

  Iodine
• What stain is used for animal cells? (ex: skin cells)

  Crystal violet
• What stain is used for plant cells? (ex: leaves)

  Malachite green
• Eyepiece (or ocular lens)


  The lens the viewer looks through to observe the specimen
• Arm


  Connects the body tube to the base of the microscope
• Base


  Supports the microscope and is where the illuminator is located
• Iris Diaphragm


  Adjusts the amount of light that reaches the specimen
• Illumination (or light source)


  Older microscopes used mirrors to reflect light from an external source up through the bottom of the stage; however, most microscopes now use a low-voltage bulb
• Stage


  The flat platform where the slide is placed
• Stage clip


  Metal clips that hold the slide in place
• Coarse focus/adjustment knob


  Brings the specimen into general focus
• Fine focus/adjustment knob


  Tunes the focus and increases the detail of a specimen
• Stage adjustment knobs


  Knobs that move the stage left and right or up and down
• Rotating nosepiece


  A rotating turret that houses the objective lenses. The viewer spins this to select different objective lenses.
• Aperture


  The hole in the middle of the stage that allows light from the illuminator to reach the specimen.
• How to focus an object under the microscope: Step 1


  Plug in the microscope and turn it on
• How to focus an object under the microscope: Step 2


  Look through the eyepiece and adjust the iris diaphragm to get a bright field of view
• How to focus an object under the microscope: Step 3


  Click the 4X objective into place using objective nosepiece
• How to focus an object under the microscope: Step 4


  Position the slide on the stage using the stage clip
• How to focus an object under the microscope: Step 5


  Use the stage adjustment knobs to center the specimen over the aperture
• How to focus an object under the microscope: Step 6


  Use the coarse adjustment knob to bring the specimen into general view
• How to focus an object under the microscope: Step 7


  Use the fine focus knob to sharpen the focus
• How to focus an object under the microscope: Step 8


  When moving to a higher objective lens, only use the fine focus knob
• total magnification = eyepiece x objective
• The image of a specimen is inverted and reversed under the microscope
• the field of view decreases as you move from low to high power objective lenses
• When an object is moved one direction, it appears to move the same direction under the microscope
• What is A?

  Base

  
• What is B?

  Illuminator

  
• What is C?

  Iris Diaphragm

  
• What is D?

  Aperture

  
• What is E?

  Objective lens

  
• What is F?

  Eyepiece

  
• What is G?

  Stage clip

  
• What is H?

  Arm

  
• What is K?

  Stage adjustment knob