Transciption

• 1. RNA polymerase binds to the DNA, causing the double helix to unwind in that location and the hydrogen bonding between the bases breaks and are separated into the coding and template strand.
• 2. This exposes the gene to be transcribed
• 3. RNA polymerase begins moving down the template DNA strand in the 3' to 5' direction.
• 4. Free nucleotides are added in sequence to their complementary base pairing on the template DNA strand. Hydrogen bonding reforms between these pairs.
• 5. The new strand of RNA is synthesised in the 5' to 3' direction.
• 6. Transcription is completed, the RNA sequence has been synthesised. RNA polymerase detaches from DNA molecules and the hydrogen bonding breaks between the RNA and DNA and the double helix reforms.
• 7. This results in mRNA being formed.
• 8. This is called pre-mature mRNA as the intron sequences are still present.
• 9. The process of splicing takes place and the introns are spliced out and the exons fuse together and form mature mRNA.
• 10. Now ready for translation the mature mRNA.