how can temperature's effect on decay be investigated?
1. measure out 5 cm³ of lipase solution and add it to a test tube. label this tube with an 'L' for lipase
2. measure out 5 cm³ of milk and add it to a different test tube
3. add 5 drops of phenolphthalein indicator to the tube containing milk
4. measure out 7 cm³ of sodium carbonate solution and add it to the tube containing milk and phenolphthalein. This makes the solution in the tube alkaline, so it should turn pink
5. put both tubes into a water bath set to 30 °C and leave them to reach the temperature of the water bath. you could stick a thermometer into the milk tube to check this
6. once the tubes have reached 30 °C, use a calibrated dropping pipette (a dropping pipette with a scale) to put 1 cm' of the lipase solution into the milk tube and start a stopwatch straight away
7. stir the contents of the tube with a glass rod. the enzyme will start to decompose the milk
8. as soon as the solution loses its pink colour, stop the stopwatch and record how long the colour change took in a table
9. repeat the experiment at a range of different temperatures (e.g. 10 °C, 20 °C, 40 °C, 50 °C). make sure you carry out the experiment three times at each temperature, then calculate the mean time taken for the colour change to occur at each temperature
10. use your results to calculate the rate of decay