1)Several groups of cells are scrapped off the leaf
2) Nutrients are add to the agar jelly
3) Hormones are added to adgar jelly
4)The plant cells are kept in sterile conditions
5)The plant cells are kept and 20 degrees
1)so that many plants can be produced
3) so roots and shoots can develop
4)to prevent microorganisms from entering
5) optimum temperature for grow