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AUBF Lab
Module 1
Polarized
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Cards (18)
In polarized microscopy, the direction of light has how many direction?
one
In polarized microscopy, how many plane of direction of vibration does it have?
one
Polarized microscopes illuminates the specimen with what type of light?
Polarized light
Polarized microscopy
: identifies and classifies
BIREFRINGENT
SUBSTANCES that shine brillianty against
DARK
background.
Birefringent
: ability of substance to refract light in 2 DIRECTIONS at 90 degrees
What are the 2 types of birefringence?
Positive
and
Negative
What is the rotation of the polarized light in positive birefringence?
Clockwise
What is the rotation of the polarized light in negative birefringence?
counterclockwise
Positive birefringence + RED COMPENSATOR is = to what color?
Blue-green
Negative birefringence + RED COMPENSATOR is = to what color?
Yellow
Give an example of positive birefringence?
Calcium pyrophosphate
Give an example of negative birefringence?
Monosodium urate
Red compensator
: indicates the type of birefringence if it is positive or negative
Polarizing
filter: below condenser
Analyzer
: between objective and eyepiece
Polarizing filter
:
east-west
direction for light vibration
Analyzer
:
north-south
direction for light vibration
Crossed position
or
crossed pols
: A position in which the poles of a magnet are in the same plane.