All chemical reactions that occur in an organism; most occur in cells and use intracellular enzymes, e.g. phagocyticwhitebloodcells use them to digest bacteria that they engulf; a way that enzymes are used as a defense mechanism
Sometimes enzymes are secreted from cells to catalyze reactions outside called extracellular enzymes e.g. in animal digestion, like mammals, within internal digestive systems
Organisms such as fungi feed as saprophytes - release digestive enzymes onto the large/insoluble food molecules and absorb the smaller/soluble products