PARA L4

• Subkingdom: Protozoa

  Phylum: Sarcomastigophora

  Subphylum: Sarcodina

  Superclass: Rhizopodia

  Order: Amoebida

  Family: Endoamoebidae

  Genus: Entamoeba, Endolimas, Iodamoeba
• active form of amoeba

  trophozoite
• inactive form of amoeba capable of protecting itself

  Cyst
• a single-celled organism that constantly changes shape

  amoeba
• genus of an amoeba where there is a small karyosome at center of nucleus, with peripheral chromatin granules at the nuclear membrane and delicate achromatic threads that connects the karyosome to nuclear membrane

  Entamoeba
• genus of an amoeba with relatively large karyosom of irregular shape, with several achromatic threads connecting the karyosome to the nuclear membrane and thin layer of peripheral chromatin which is inconspicuous

  Endolimax
• genus of an amoeba with large karyosome rich in chromatin and surrongded by a single layer of periendosomal granules which are attches to karyosome and nuclear membrane by the radiating achromatic granules

  iodamoeba
• non invasive kind of E.hist

  Entamoeba dispar
• small rays of the E.hist

  Entamoeba hartmani
• trophozoite size of Entamoeba histolytica

  10-60 um
• cyst size of Entamoeba histolytica

  5-20 um
• peripheral chromatin of E. hist

  fine and evenly distributed
• cytoplasmic inclusions of E.hist trophozoite

  ingested RBCs
• cytoplasmic inclusions of E.hist cyst

  chromatoid bar, round end and diffuse glycogen mass
• appearance of the cytoplasm of the E.hist trophozoite


  ground glass
• it measures the pathogenesity of the strain of amoeba

  isozyme analysis
• infection of E.hist to man

  mature quadranucleated cyst
• habitat of the E.hist

  Small intestine
• this can rupture the cyst wall

  tripsin
• cyst remains variable at:

  • damp soil (8days)
  • moist soil (12 days)
  • H2O (9-30 days)
  • H2O at 4c (3 months)
• mechanical vectors of the E.hist that remains variable at 48 hrs

  • Filth flies
  • mooska dosmestica
  • cockroach
• pathogenesity of the E. hist

  non-invasive infection

  intestinal disease

  Extra-intestinal disease
• stool exam of the intestinal amoebiasis

  3/4 days interval, direct smear andt stained mounts
• preservatives of a watery stool of E.hist

  MIF

  PVA - perma stain

  Scaudin's fixative - perma stain

  IH/ trichrome stain - perma mount
• a diagnosis of E.hist which is collected by a physician, 1/3 lesion,

  sigmoidscophy material
• a diagnosis used for studying metabolism, pathogenesity and production of antigens for serodiagnosis

  culture
• what are the culture diagnosis of the E.hist

  Dibasic medium of Boeck and Drbohlav

  Diamond's medium (TYI-S-33)
• what are the diagnosis of the intestinal amoebiasis of E.hist


  Stool examination

  Sigmoidscopy material

  Culture

  Seroimmunologic test
• treatment of the asymptomatic E.hist

  P - paromomycin

  M - metrodanizole

  D - Diloxomide furoate
• treatment for the symptomatic intestinal amoebiasis

  P - paromomycin

  I - iodoquinol

  D - diloxamide furoate
• treatment for extra-intestinal amoebiasis

  M - metrodanizole

  T - tinidazole
• size of E.coli trophozoite

  15-50 um
• size of E.coli cyst

  10-33 um
• peripheral chromatin of E.coli

  unevenly distributed
• cytoplasm of E.coli

  coarse and granulated
• cytoplasmic inclusions of E.coli trophozoite

  ingested bacteria
• cytoplasmic inclusion of the E.coli cyst

  thin chromatoid bars with splintered ends
• size of the E. hart trophozoite

  5-15 um
• size of E.hart cyst

  5-12 um
• peripheral chromatin of E.hart

  fine and evenly distributed