Describe a method to investigate the effect of pH on amylase.
1) Place 1 drop of iodine solution into each well of a spotting tile.
2) In the first test tube, add 2cm3 of starch solution.
3) In the second test tube, add 2cm3 of amylase solution.
4) In the third test tube, add 2cm3 of pH5 buffer solution.
5) Place all 3 test tubes in a water bath (30°C) for 10min to allow the solutions to reach the correct temperature.
6) Combine the 3 solutions into 1 test tube and mix with a stirring rod. Return test tube to the waterbath and start a stopwatch.
7) After every 30s, use the stirring rod to transfer 1 drop of solution into a spotting tile well.
8) Iodine should turn blue-black if starch is present.
9) Stop transferring when the iodine remains orange. (reaction complete). record this time.
10) Repeat whole experiment with different pH buffers.