H^+ ions/protons interfere with hydrogen and ionic bonds
Changing concentration
Affects 3D tertiary structure and active site
Changes reaction rate
Extreme: Irreversible shape change (denatured)
Experiment: Maintain pH by adding set pH buffer volume
Induced fit model:
Charged groups at active site
Important for binding substrate
Increasing H^+ ion concentration change active site charge
Reduce ESC formation
pH graph:
Symmetrical
Bell-shaped
Most intracellular enzymes are neutral (optimum pH = 7), not all
