method effect of pH the rate of react amalys
1. Set up test tubes with different pH solutions and a set volume of starch solution
2. Put in water bath at 30 oC, leave for 10 mins
3. Set up spotting tiles with 1 drop of iodine solution in each well
4. Add a set volume & concentration of amylase to one of test tubes, start stopwatch / timer
5. Remove a drop of amylase-starch mixture every 30 seconds and place into a spotting tile to test for starch (blue-black)
6. Record time when no starch is detected (no longer turns blue-black)
7. Repeat steps 4 - 6 for each pH buffer solution