dna sequencing

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Created by
Angad

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  • DNA sequencing involves determining the exact nucleotide sequence (order of bases) in a DNA molecule. This can be used to sequence a specific gene on a chromosome or the genome
  • The data is utilised to:
    • study evolutionary relationships between species
    • study the inheritance of diseases 
    • model changes in the frequencies of genes and alleles in populations
  • sanger method
    PCR is utilised to produce many copies of the target DNA. The PCR mixture contains fluorescent ‘chain terminator’ nucleotides to terminate replication so that the resulting length of a DNA sequence will reflect the specific nucleotide position marking the end of the fragment.  The fragments are separated using gel electrophoresis. This allows the base sequence to be determined by ordering the fragments according to length.