When insect is less active, tracheoles ends contain fluid
As activity increases, fluid is removed
Muscle cells produce lactic acid
Lowers cell’s water potential
Gas exchange is near cells, quicker
When cells actively respire
O_2 is used up
So its concentration at tracheoles end falls
Creating diffusion gradient for O_2 towards cells
CO_2 produced by respiring cells
Creates diffusion gradient with atmosphere, it move out